A discontinuous buffer system for paper electrophoresis of human hemoglobins.

NEW BUFFER SYSTEM has recently been introduced for the separation of proteins (1) and hemoglobins (2) on starch gel. It differs from the conventional barbital system most frequently used heretofore in that the buffer used for the supporting medium differs from that used in the buffer vessels. For this reason the buffer system is referred to as discontinuous. The discontinuous buffer system appears to be superior not only for electrophoretic separations using starch gel as a supporting medium (2) but for electrophoresis on paper as well. A method for paper electrophoresis of hemoglobins will be described, in which Tris-EDTA-borate buffer is used for the immersion of the paper and barbital buffer is used in the buffer vessels.